Influenza b virus mutants and uses therefor
AssigneeFluGen, Inc.
InventorsMichael J. Moser, Yasuko Hatta, Pamuk Bilsel
Life of this patent
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Abstract
Disclosed herein are compositions and methods related to mutant viruses, and in particular, mutant influenza viruses. The mutant viruses disclosed herein include a mutant BM2 sequence, and are useful in immunogenic compositions, e.g., as vaccines. Also disclosed herein are methods, compositions and cells for propagating the viral mutants, and methods, devices and compositions related to vaccination.
Claims8
-19. (canceled)
A method for propagating a recombinant influenza A virus, comprising:
- contacting a host cell with a recombinant influenza A virus comprising SEQ ID NO:
- 33;
- and incubating the host cell for a sufficient time and under conditions suitable for viral replication, wherein the host cell is an MDCK cell modified to produce a wild-type version of the influenza BM2 gene, thereby providing a BM2 gene product to the virus in trans.
A method for propagating a recombinant influenza A virus comprising:
- contacting a host cell with a recombinant influenza A virus comprising SEQ ID NO:
- 33;
- and incubating the host cell for a sufficient time and under conditions suitable for viral replication, wherein the host cell is a Vero cell modified to produce a chimeric version of the influenza A M2 and influenza B BM2 gene selected from the group consisting of SEQ ID NO:
- 28, SEQ ID NO:
- 29, SEQ ID NO:
- 30, SEQ ID NO:
- 31, and SEQ ID NO:
- 32, thereby providing a chimeric M2:BM2 gene product to the virus in trans.
A method for propagating a recombinant influenza A virus comprising:
- contacting a host cell with a recombinant influenza A virus comprising SEQ ID NO:
- 33;
- and incubating the host cell for a sufficient time and under conditions suitable for viral replication, wherein the host cell is a Vero cell modified to produce a codon-optimized version of the BM2 gene comprising SEQ ID NO:
- 27, thereby providing a BM2 gene product to the virus in trans.
A recombinant influenza A virus comprising a mutant BM2 protein.
A host cell for propagating a recombinant influenza virus, wherein the host cell is a Vero cell modified to produce a gene product encoded by a cDNA sequence selected from the group consisting of SEQ ID NO:
- 27, SEQ ID NO:
- 28, SEQ ID NO:
- 29, SEQ ID NO:
- 30, SEQ ID NO:
- 31, and SEQ ID NO:
- 32.
Dependencies are inferred from the claim text.