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US 2024/0091165 A1Published application
Melt processed viral nanoparticle constructs
AssigneeCase Western Reserve University
InventorsNicole F. Steinmetz, Jonathan Pokorski
Life of this patent
Estimating the term…
Abstract
A melt processed viral nanoparticle construct for delivery of virus or virus-like particles to a site of interest includes a degradable polymer matrix and a plurality of virus or virus-like particles encapsulated within the degradable polymer matrix. The nanoparticle construct upon administration to the site of interest providing a sustained release of the virus or virus-like particles and/or nanoparticles upon degradation of the polymer matrix.
Claims24
1:
- A melt processed degradable viral nanoparticle construct for delivery of virus or virus-like particles to a site of interest, the nanoparticle construct comprising:
- a degradable polymer matrix, and
- a plurality of virus or virus-like particles encapsulated within the degradable polymer matrix, the nanoparticle construct upon administration to the site of interest providing a sustained release of the virus or virus-like particles upon degradation of the polymer matrix, the virus or virus-like particles upon release from the degradable polymer matrix having the same or substantially similar structural and/or biochemical characteristics as the virus or virus-like particles prior to melt processing.
2:
- The nanoparticle construct of claim 1, wherein the degradable polymer matrix comprises a melt processable degradable polymer material that is biocompatible and, upon degradation, produces substantially non-toxic products.
3:
- The nanoparticle construct of claim 2, wherein the site of interest is a cell or tissue of a subject.
4:
- The nanoparticle construct of claim 2, wherein the site of interest is a plant propagation material, a plant, part of a plant and/or plant organ.
5:
- The nanoparticle construct of claim 2, wherein the melt processable degradable polymer material is a melt processable biodegradable polymer.
6:
- The nanoparticle construct of claim 3, the virus or virus-like particles having a release profile from the degradable polymer matrix at least partially defined by the degradation of the degradable polymer material under physiological conditions.
7:
- The nanoparticle construct of claim 2, wherein the melt processing of degradable polymer material and the virus or virus-like particles is at a Peclet number of about 5 to about 25, wherein the Peclet number is determined by the following equation:
- P
-
- e
- =
- 6
-
- πη
-
- γ
- .
-
- R
- 3
- k
- b
-
- T
- where:
- η=viscosity of the polymer melt (Pa·s)
- {dot over (γ)}=shear rate applied to the system (s−1)
- R=weight average radius of the particles before shear application (m)
- kb=Boltzmann's constant (J·K−1)
- T=temperature of the system (K).
21 more elements
8:
- The nanoparticle construct of claim 1, wherein the virus or virus-like particles are substantially uniformly dispersed in the degradable polymer matrix.
9:
- The nanoparticle construct 2, wherein the degradable polymer material has melt temperature below the degradation temperature of the virus or virus-like particles.
10:
- The nanoparticle construct of claim 2, wherein the degradable polymer material comprises poly(lactic-co-glycolic acid) (PLGA) or a copolymer thereof.
11:
- The nanoparticle construct of claim 1, further comprising at least one porogen.
(canceled)
13:
- The nanoparticle construct of claim 1, wherein the virus or virus-like particle is a bacteriophage or plant virus or virus like-particle.
14:
- The nanoparticle construct of claim 1, wherein the virus or virus-like particle is a plant picornavirus or a filamentous plant virus or virus-like particle.
(canceled)
16:
- The nanoparticle construct of claim 13, wherein the plant virus or virus-like particle is a Qβ bacteriophage or virus-like particle, a cowpea mosaic virus-like particle or potato virus X virus-like particle.
17:
- The nanoparticle construct of claim 13, wherein the plant virus particle or virus-like particle is a rod-shaped virus particle.
(canceled)
19:
- The nanoparticle construct of claim 1, wherein the virus or virus-like particle is loaded with or bonded to a cargo molecule.
20:
- The nanoparticle construct of claim 19, wherein the cargo molecule comprises at least one of a therapeutic agent, detectable moiety, or targeting agent.
21:
- The nanoparticle construct of claim 20, the therapeutic agent comprising an anticancer agent.
22:
- The nanoparticle construct of claim 1, providing in situ delivery of the virus or virus-like particles upon administration to a subject.
23:
- The nanoparticle construct of claim 1, being provided in the shape of a plurality of microparticles or microneedles.
24-75. (canceled)
Dependencies are inferred from the claim text.