Split-intein methods and compositions for generation of single sex offspring
OwnerJoseph Fenton LAWLERinventor
Life of this patent
Estimating the term…
Abstract
Described herein are methods and compositions for generating single sex offspring using split-intein approach. In particular, methods and compositions are provided to generate single sex and genetically modified offspring. These techniques can be applied to compassionate animal breeding.
Claims26
-88. (canceled)
A method of producing a single sex population of non-human vertebrate animals, the method comprising:
- crossing (i) a first non-human vertebrate animal having a first genotype comprising a pair of heterozygous chromosomes comprising one or more nucleotide sequences encoding a first toxin fragment with (ii) a second non-human vertebrate animal having a second genotype comprising a pair of homozygous chromosomes comprising one or more nucleotide sequences encoding a second toxin fragment;
- wherein a resulting progeny having a genotype comprising the first toxin fragment and the second toxin fragment is not viable;
- thereby creating a single sex population.
(canceled)
A method of producing a single sex population of non-human vertebrate animals, comprising the steps of:
- (a) obtaining (i) a first non-human vertebrate animal, wherein one or more chromosomes comprise a first expression cassette comprising the following elements in 5′ to 3′ orientation:
- a first promoter, operatively linked thereto a nucleotide sequence encoding for a first toxin fragment and a first split intein fragment;
- (b) obtaining (ii) a second non-human vertebrate animal, wherein one or more allosomes comprise a second expression cassette comprising the following elements in 5′ to 3′ orientation:
- a second promoter, operatively linked thereto a nucleotide sequence encoding for a second toxin fragment and a second split intein fragment;
- (c) crossing the first non-human vertebrate animal and the second non-human vertebrate animals, wherein a resulting progeny containing the first expression cassette and the second expression cassette is not viable;
- thereby creating a single sex population.
(canceled)
-107. (canceled)
-118. (canceled)
A method of producing a single sex population of non-human vertebrate animals, comprising the steps of:
- (a) obtaining (i) a first non-human vertebrate comprising one or more nucleotide sequences integrated in one or more chromosomes comprises the following elements in 5′ to 3′ orientation:
- a first inactive promoter, operatively linked thereto a nucleotide sequence encoding for a toxin;
- (b) obtaining (ii) a second non-human vertebrate animal comprising one or more nucleotide sequences integrated in one or more chromosomes comprising the following elements in 5′ to 3′ orientation:
- a second promoter, operatively linked thereto a transcription factor corresponding to the inactive promoter;
- and
- (c) crossing the first non-human vertebrate animal and the second non-human vertebrate animals, wherein a resulting progeny, containing the first inactive promoter, the second promoter expressing the transcription factor corresponding to the first inactive promoter, and the corresponding transcription factor, is not viable;
- thereby creating a single sex population.
(canceled)
Dependencies are inferred from the claim text.