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US 2024/0084359 A1Published application
Methods and compositions for patterned molecular array generation by directed bead delivery
Assignee10x Genomics, Inc.
InventorsMichael SCHNALL-LEVIN, Preyas SHAH
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Abstract
Provided in some aspects are methods of patterning a surface in situ for producing an array on the surface, for example, by partitioning of beads comprising oligonucleotides into spatially predefined regions, to generate unique DNA sequences in spatial positions in the array. Compositions such as nucleic acid arrays produced by the methods are also disclosed.
Claims21
1-69. (canceled)
A method for providing an array, comprising:
- (a) partitioning a plurality of Round 1 beads into wells on a substrate, wherein the Round 1 bead in a first well and the Round 1 bead in a second well each comprises a different Round 1 oligonucleotide;
- (b) disrupting the Round 1 beads to release the Round 1 oligonucleotides, wherein the released Round 1 oligonucleotides are attached to nucleic acid molecules in the corresponding well via ligation to generate extended nucleic acid molecules;
- (c) partitioning a plurality of Round 2 beads into wells on the substrate, wherein the Round 2 bead in the first well and the Round 2 bead in the second well each comprises a different Round 2 oligonucleotide;
- and
- (d) disrupting the Round 2 beads to release the Round 2 oligonucleotides, wherein the released Round 2 oligonucleotides are attached to the extended nucleic acid molecules in the corresponding well via ligation to generate further extended nucleic acid molecules.
2 more elements
Dependencies are inferred from the claim text.