Microbial polysaccharides and methods of use
AssigneeMorgan State University
InventorsPumtiwitt McCarthy, Sujan Ghimire
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Abstract
Methods for removing heavy metals from contaminated water including contacting contaminated water with polysaccharides from N. meningitides serotypes B and W; a fusion gene product and fusion enzyme including silica acid synthase and CMP sialic acid synthetase, and use of the fusion enzyme in a simplified process to make CMP Sialic acid and derivatives thereof. Use of CMP Sialic acid and derivatives thereof to remove heavy metals from contaminated water.
Claims5
A method of removing heavy metals from contaminated water comprising passing said contaminated water over an inert water-insoluble substrate to which is bound a compound selected from the group consisting of capsular polysaccharide of N. meningitidis serogroup B, capsular polysaccharide of N. meningitidis serogroup W, CMP-Sialic acid, derivatives thereof, and combinations thereof.
A DNA molecule comprising the DNA sequence of sialic acid synthase and the DNA sequence of CMP-Sialic acid synthetase.
A method of synthesizing CMP-Sialic acid comprising incubating, in a single reaction vessel without isolating intermediates, a fusion protein comprising the amino acid sequences of sialic acid synthase and CMP-Sialic acid synthetase with N-Acetylmannosamine, phosphoenolpyruvate and cytidine triphosphate.
A method of synthesizing derivatives of CMP-Sialic acid comprising incubating, in a single reaction vessel without isolating intermediates, a fusion protein comprising the amino acid sequences of sialic acid synthase and CMP-Sialic acid synthetase with analogs of N-Acetylmannosamine, phosphoenolpyruvate and cytidine triphosphate.
Dependencies are inferred from the claim text.