Recombinant expression vector for production of encapsulin-based vaccine and method for manufacturing the same
AssigneeInthera Inc.
InventorsDeog Young CHOI, Hyun Gyo JUNG
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Abstract
The present invention relates to an encapsulin protein and a fusion protein comprising the same, and more specifically to a recombinant expression vector for vaccine production, and a preparation method therefor, the vector comprising polynucleotides that encode a target protein, an encapsulin protein and an RNA interacting domain (RID) protein, so as to improve the expression efficiency of the target protein, and thus enables a water-soluble vaccine to be produced in a highly efficient manner and a large target protein to be used.
Claims21
A recombination expression vector for vaccine production, comprising polynucleotides that encode:
- a target protein;
- an encapsulin protein;
- and
- an RNA interacting domain (RID) protein.
A fusion protein, comprising:
- a target protein;
- an encapsulin protein;
- and an RNA interacting domain (RID) protein.
A method for producing a vaccine, comprising the steps of:
- a) producing a recombinant expression vector for vaccine production comprising polynucleotides that encode a target protein;
- an encapsulin protein;
- and an RID protein;
- b) producing a transformant by introducing the recombinant expression vector into a host cell;
- and
- c) culturing the transformant to induce the expression of a recombinant fusion protein, and obtaining the same.
Dependencies are inferred from the claim text.