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AssigneeUniversite De Geneve
InventorsDaniel David Pinschewer, Doron Merkler, Sandra Margarete Kallert, Mario Kreutzfeldt, Stéphanie Gabrielle Darbe Abdelrahman, Nicolas Jean Page
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The present application relates to arenaviruses with rearrangements of their open reading frames (“ORF”) in their genomes. In particular, described herein is a modified arenavirus genomic segment, wherein the arenavirus genomic segment is engineered to carry a viral ORF in a position other than the wild-type position of the ORF. Also described herein are trisegmented arenavirus particles comprising one L segment and two S segments or two L segments and one S segment. The arenavirus, described herein may be suitable for vaccines and/or treatment of diseases and/or for the use in immunotherapies.
An arenavirus genomic segment, wherein the genomic segment is engineered to carry a viral open reading frame (“ORF”) in a position other than the wild-type position of the ORF, wherein the arenavirus genomic segment is selected from the group consisting of:
A tri-segmented arenavirus particle comprising one L segment and two S segments, wherein propagation of the tri-segmented arenavirus particle does not result in a replication-competent bi-segmented viral particle after 70 days of persistent infection in mice lacking type I interferon receptor, type II interferon receptor and recombination activating gene 1 (RAG1) and having been infected with 104 PFU of the tri-segmented arenavirus particle.
A tri-segmented arenavirus particle comprising two L segments and one S segment, wherein propagation of the tri-segmented arenavirus particle does not result in a replication-competent bi-segmented viral particle after 70 days of persistent infection in mice lacking type I interferon receptor, type II interferon receptor and recombination activating gene 1 (RAG1) and having been infected with 104 PFU of the tri-segmented arenavirus particle.
A tri-segmented arenavirus particle comprising one L segment and two S segments, wherein a first S segment is engineered to carry an ORF encoding GP in a position under control of an arenavirus 3′ UTR and an ORF encoding a first gene of interest in a position under control of an arenavirus 5′ UTR and a second S segment is engineered to carry an ORF encoding NP in a position is under control of an arenavirus 3′ UTR and an ORF encoding a second gene of interest in a position under control of an arenavirus 5′ UTR.
A tri-segmented arenavirus particle comprising one L segment and two S segments, wherein a first S segment is engineered to carry an ORF encoding GP in a position under control of an arenavirus 5′ UTR and an ORF encoding a first gene of interest in a position under control of an arenavirus 3′ UTR and a second S segment is engineered to carry an ORF encoding NP in a position is under control of an arenavirus 5′ UTR and an ORF encoding a second gene of interest in a position under control of an arenavirus 3′ UTR.
Dependencies are inferred from the claim text.