Compounds, Kits, and Methods for Bead-based Detection of Anti-microbe Antibodies
OwnerMary Lape Nixoninventor
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Abstract
The present disclosure relates generally an immunoassay platform that utilizes a bead-based system to detect the presence or absence of antibodies in a test sample. The disclosed platform relates to methods and kits and can be used to detect the presence, absence, or exposure to Borrelia miyamotoi or Hepatitis D virus (HDV), or other pathogens or microbes of interest in a subject.
Claims60
A method of determining exposure of a subject to a pathogen or microbe, comprising (i) incubating a liquid biological sample obtained from a subject with a bead particle comprising a core bead coupled to a plurality of antibodies bound to an antigen derived from a pathogen or microbe of interest, such that the antigen is presented on the outside of the bead particle and any antibodies in the liquid biological sample that recognize the antigen can bind to the bead particle;
- (ii) washing the bead particle;
- (iii) incubating the bead particle with a detectably labeled antibody that binds to an antibody of the species of the subject;
- and determining the presences or absence of the detectably labeled antibody, wherein the presence of the detectable label indicates the subject was exposed to the pathogen or microbe of interest and the absence of the detectable label indicates the subject was not exposed to the pathogen or microbe of interest.
A bead particle comprising a microparticle coupled to at least one antibody or a plurality or antibodies bound to an antigen derived from a pathogen or microbe of interest;
- wherein the antigen comprises a tag sequence and wherein the antibody or antibodies specifically bind to the tag sequence.
A method of determining exposure of a subject to Borrelia miyamotoi, comprising (i) incubating a liquid biological sample obtained from a subject with a bead particle comprising a core bead coupled to a plurality of antibodies bound to a B. miyamotoi glycerophosphodiester phosphodiesterase (glpQ), such that any anti-glpQ antibodies in the liquid biological sample can bind to the glpQ on the bead particle;
- (ii) washing the bead particle;
- (iii) incubating the bead particle with a detectably labeled antibody that binds to an antibody of the species of the subject;
- and determining the presences or absence of the detectably labeled antibody, wherein the presence of the detectable label indicates the subject was exposed to B. miyamotoi and the absence of the detectable label indicates the subject was not exposed to B. miyamotoi.
A bead particle comprising a microparticle coupled to a plurality of antibodies bound to a B. miyamotoi glycerophosphodiester phosphodiesterase (glpQ).
A method of determining exposure of a subject to hepatitis D virus (HDV), comprising (i) incubating a liquid biological sample obtained from a subject with a bead particle comprising a core bead coupled to a plurality of antibodies bound to a HDV antigen, such that any antibodies in the liquid biological sample that are capable of binding to the HDV antigen can bind to the HDV antigen on the bead particle;
- (ii) washing the bead particle;
- (iii) incubating the bead particle with a detectably labeled antibody that binds to an antibody of the species of the subject;
- and determining the presences or absence of the detectably labeled antibody, wherein the presence of the detectable label indicates the subject was exposed to HDV and the absence of the detectable label indicates the subject was not exposed to HDV.
A bead particle comprising a microparticle coupled to a plurality of antibodies bound to a hepatitis D virus (HDV) antigen.
Dependencies are inferred from the claim text.