Methods and compositions for incorporating nucleotides
AssigneeIsoPlexis Corporation
InventorsSteven GORDON, Jerzy OLEJNIK
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Abstract
The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
Claims2
A method for determining an identity of a nucleic acid at an interrogation position in a nucleotide sequence from data acquired from one or more channels, comprising
- a) obtaining a data set for one or more probe intensities at one or more nucleic acid positions in said sequence, wherein each probe corresponds to a nucleic acid,
- b) determining the ratio contribution to probe intensity at said interrogation position from probe intensities at the interrogation position and at one or both of
- i) at least one subsequent nucleic acid positions in said sequence, and
- ii) at least one preceding nucleic acid positions in said sequence, and
- c) applying said ratio contribution to probe intensity to said data set to arrive at an identity for a nucleic acid at said interrogation position in said nucleotide sequence.
A method for field flattening an image of a probe on a solid support, comprising
- a) obtaining a first data set for a plurality of pixel intensities of a first raw image of a probe at a first concentration on a solid support, wherein said first raw image is produced using a first spectral filter for detecting a first probe,
- b) obtaining a second data set for a plurality of pixel intensities of a second smoothed image of said probe on said solid support, wherein said second smoothed image is produced using a low-pass filter,
- c) determining a field flattening intensity value for a plurality of pixels of said first raw image, and
- d) generating a third field flattened image of said probe on said solid support using said field flattening intensity of said plurality of pixels, wherein the correlation of intensity of a plurality of pixels to their spatial location on said third field flattened image is reduced compared to the intensity of a plurality of pixels at a corresponding location on said first raw image.
Dependencies are inferred from the claim text.