Lambda-carrageenase mutant ouc-cgla-dpqq and application thereof

Abstract

The present disclosure discloses λ-carrageenase mutant OUC-CglA-DPQQ, which has an amino acid sequence as set forth in SEQ ID NO. 1 and an encoding gene as set forth in SEQ ID NO. 2. The λ-carrageenase mutant OUC-CglA-DPQQ is applied to degradation of carrageenan/preparation of λ-carrageenan oligosaccharides with high polymerization degree. The present disclosure further discloses a method for degrading carrageenan/preparing λ-carrageenan oligosaccharides with high polymerization degree by using the λ-carrageenase mutant OUC-CglA-DPQQ. The λ-carrageenase mutant OUC-CglA-DPQQ of the present disclosure can act on low viscosity carrageenan, and the polymerization degree of final product λ-carrageenan oligosaccharides is 10-20. The λ-carrageenase mutant of the present disclosure is excellent in enzymatic properties and specificity, and has important industrial application value and economic value in the preparation of λ-carrageenan oligosaccharides via an enzyme method.

Dependencies are inferred from the claim text.