Aconitic acid exporter (aexa) increases organic acid production in aspergillus
AssigneeBattelle Memorial Institute
InventorsShuang Deng, Jon K. Magnuson, Joonhoon Kim, Kyle R. Pomraning, Ziyu Dai, Beth A. Hofstad
Life of this patent
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Abstract
Recombinant Aspergillus genetically modified to increase expression of g8846, renamed herein as aconitic acid exporter (aexA), are provided, which in some examples are also genetically inactivated for an endogenous cis-aconitic acid decarboxylase (cadA) gene. Such recombinant Aspergillus produce more aconitic acid as compared to native Aspergillus. Also provided are methods of using such recombinant Aspergillus to increase production of aconitic acid and other organic acids, such as citric acid, itaconic acid, and 3-hydroxypropionic acid (3-HP).
Claims19
A method of producing 3-hydroxypropionic acid (3-HP), comprising:
- culturing an isolated recombinant Aspergillus fungus under conditions that permit the fungus to produce 3-HP, thereby making 3-HP, wherein the fungus comprises:
- an exogenous nucleic acid molecule encoding aconitic acid exporter (aexA) operably linked to an exogenous promoter, thereby overexpressing the aexA in the fungus;
- an endogenous or exogenous nucleic acid molecule encoding aspartate 1-decarboxylase (panD);
- an endogenous or exogenous nucleic acid molecule encoding β-alanine-pyruvate aminotransferase (BAPAT);
- and
- an endogenous or exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (3-HPDH).
A method of making citric acid, comprising:
- culturing an isolated recombinant Aspergillus fungus under conditions that permit the fungus to produce citric acid, thereby making citric acid, wherein the fungus comprises:
- an exogenous nucleic acid molecule encoding aconitic acid exporter (aexA) operably linked to an exogenous promoter, thereby overexpressing the aexA in the fungus.
A method of making itaconic acid, comprising:
- culturing an isolated recombinant Aspergillus fungus under conditions that permit the fungus to produce itaconic acid, thereby making itaconic acid, wherein the fungus comprises:
- an exogenous nucleic acid molecule encoding aconitic acid exporter (aexA) operably linked to an exogenous promoter, thereby overexpressing the aexA in the fungus.
Dependencies are inferred from the claim text.