Materials and methods for brewing beer
AssigneeOmega Yeast Labs, LLC
InventorsLaura Burns, Lance Shaner
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Abstract
Described herein are materials and methods for the conversion of a non-volatile form of 3-sulfanyl-1-hexanol (3SH) to free 3SH during a brewing process.
Claims69
A recombinant Saccharomyces spp comprising a polynucleotide encoding a yeast β-lyase enzyme Irc7 operably linked to a heterologous promoter, wherein the β-lyase enzyme comprises an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:
- 1.
A method of converting of a non-volatile form of 3-sulfanyl-1-hexanol (3SH) to free 3SH during a brewing process, the method comprising
- (a) a mash hopping step comprising adding a plant comprising a non-volatile form of 3SH to mash to produce wort;
- (b) boiling the wort produced by (a);
- (c) cooling the wort;
- and
- (d) contacting the cooled wort with a recombinant Saccharomyces for a time sufficient to convert a non-volatile form of 3SH to free 3SH, wherein the recombinant Saccharomyces comprises:
- (i) a polynucleotide encoding an active β-lyase enzyme Irc7 operably linked to a heterologous promoter;
- or
- (ii) a polynucleotide encoding a cysteine-thiol lyase operably linked to a heterologous promoter.
A recombinant yeast comprising a polynucleotide encoding a yeast β-lyase enzyme Irc7 operably linked to a heterologous promoter, wherein the β-lyase enzyme comprises an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:
- 1.
The recombinant yeast of any one of claims 48-50, wherein the yeast is Hanseniaspora uvarum, Hanseniaspora guillermondii, Hanseniaspora vinae, Metschnikowia pulcherrima, Kluyveromyces/Lachancea thermotolerans, Starmerella bacillaris (previously referred to as Candida stellatal or Candida zemplinina), Saccharomycodes ludwigii, Zygosaccharomyces rouxii, Dekkera bruxellensis, Dekkera anomala, Brettanomyces custersianus, Brettanomyces naardenensis, Brettanomyces nanus, Wickerhamomyces anomalus, or Torulaspora delbrueckii.
A recombinant yeast comprising a polynucleotide encoding a cysteine-thiol lyase operably linked to a heterologous promoter.
The method of claim 64, wherein the fermentable sugar source is wort.
Dependencies are inferred from the claim text.