Method for production of moth pheromones in yeast
AssigneeDanmarks Tekniske Universitet
InventorsIrina Borodina, Carina Holkenbrink, Marie Inger Dam, Christer Löfstedt, Baojian Ding, Hong-Lei Wang
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to methods for production of (Z)-11-hexadecen-1-ol in a yeast cell. Also disclosed are methods for production of (Z)-11-hexadecenal in a yeast cell. Also disclosed are methods for production of (Z)-11-hexadecen-1-yl acetate in a yeast cell. The disclosure also provides for nucleic acid constructs and yeast cells useful for performing the present methods, as well as to pheromone compositions.
Claims20
A method for production of a moth pheromone in a yeast cell, said method comprising the steps of:
- i) providing a yeast cell capable of synthesising hexadecanoyl-CoA, said yeast cell expressing:
- a Δ11-desaturase selected from the group consisting of a Δ11-desaturase comprising SEQ ID NO:
- 2, a Δ11-desaturase comprising SEQ ID NO:
- 41, a Δ11-desaturase comprising SEQ ID NO:
- 43, and a Δ11-desaturase comprising SEQ ID NO:
- 45 or a Δ11-desaturase variant having at least 85% sequence identity to the polypeptide of SEQ ID NO:
- 2, SEQ ID NO:
- 41, SEQ ID NO:
- 43, or SEQ ID NO:
- 45, said Δ11-desaturase variant having Δ11-desaturase activity, and
- an alcohol-forming fatty acyl-CoA reductase (FAR) selected from the group consisting of a FAR comprising SEQ ID NO:
- 8, a FAR comprising SEQ ID NO:
- 16, and a FAR comprising SEQ ID NO:
- 12 or a FAR variant having at least 85% sequence identity to the polypeptide of SEQ ID NO:
- 8, or SEQ ID NO:
- 12 or a FAR variant having at least 99% sequence identity to the polypeptide of SEQ ID NO:
- 16, said FAR variant having alcohol-forming fatty acyl-CoA reductase activity;
- ii) expressing said Δ11-desaturase and said FAR from said yeast cell;
- and
- iii) incubating said yeast cell in a medium,
- whereby
- the Δ11-desaturase is capable of converting at least part of said hexadecanoyl-CoA to (Z)11-hexadecenoyl-CoA;
- and
- said FAR is capable of converting at least part of said (Z)11-hexadecenoyl-CoA to (Z)-11-hexadecen-1-ol,
- thereby obtaining a moth pheromone comprising (Z)-11-hexadecen-1-ol with a titre of at least 1 mg/L, wherein the titre is measured in a culture medium after culturing the yeast cell.
A yeast cell capable of synthesising hexadecanoyl-CoA, said yeast cell further capable of expressing:
- a Δ11-desaturase selected from the group consisting of a Δ11-desaturase comprising SEQ ID NO:
- 2, a Δ11-desaturase comprising SEQ ID NO:
- 41, a Δ11-desaturase comprising SEQ ID NO:
- 43, and a Δ11-desaturase comprising SEQ ID NO:
- 45 or a Δ11-desaturase variant having at least 85% sequence identity to the polypeptide of SEQ ID NO:
- 2, SEQ ID NO:
- 41, SEQ ID NO:
- 43, or SEQ ID NO:
- 45, said Δ11-desaturase variant having Δ11-desaturase activity, and
- an alcohol-forming fatty acyl-CoA reductase (FAR) selected from the group consisting of a FAR comprising SEQ ID NO:
- 8, a FAR comprising SEQ ID NO:
- 16, and a FAR comprising SEQ ID NO:
- 12 or a FAR variant having at least 85% sequence identity to the polypeptide of SEQ ID NO:
- 8, or SEQ ID NO:
- 12 or a FAR variant having at least 99% sequence identity to the polypeptide of SEQ ID NO:
- 16, said FAR variant having alcohol-forming fatty acyl-CoA reductase activity.
Dependencies are inferred from the claim text.