Assays, kits and methods for detection of bovine respiratory disease complex-associated pathogens
AssigneePurdue Research Foundation
InventorsMohit VERMA, Suraj MOHAN, Ana PASCUAL-GARRIGOS, Haley BROUWER, Jennifer KOZIOL, Jon SCHOONMAKER, Timothy JOHNSON
Life of this patent
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Abstract
Assays, kits, and methods that target and/or detect the presence of bovine respiratory complex (BRD)-associated pathogens in a sample. These assays, kits and methods can be portable and capable of providing fast (within 45 minutes) and accurate (at least 96%) results in the field, eliminating the need for a laboratory and other complex equipment.
Claims30
A loop-mediated isothermal amplification (LAMP) assay comprising at least one LAMP primer set that targets a deoxyribonucleic acid (DNA) fragment of a pathogen associated with a bovine respiratory disease (BRD) in a sample, wherein the assay allows for single-step identification of the pathogen.
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A method for identification of a pathogen associated with a bovine respiratory disease (BRD) in a sample and treatment thereof comprising:
- providing at least one LAMP primer set that targets a deoxyribonucleic acid (DNA) fragment of a targeted pathogen associated with a bovine respiratory disease (BRD) in a sample;
- obtaining a sample from a subject;
- combining the sample and the at least one LAMP primer set into a mixture;
- heating the combination to initiate amplification of the targeted DNA fragment;
- and
- detecting a visual result in the heated combination indicative of the presence or absence of the targeted pathogen for BRD in the sample;
- wherein the at least one LAMP primer set is one or more of primer sets A-AAAA in Tables 2 and 3.
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A kit comprising:
- at least one LAMP primer set that targets a deoxyribonucleic acid (DNA) fragment of a pathogen associated with a bovine respiratory disease (BRD) in a sample;
- at least one swab for obtaining the sample;
- and
- a heating element to initiate amplification of the targeted DNA fragment when the at least one LAMP primer set and the sample are combined.
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Dependencies are inferred from the claim text.