Bacillus cell with reduced lipase and/or esterase side activities
AssigneeBASF SE
InventorsStefan Jenewein, Max Fabian Felle, Christopher Sauer, Paul Igor Costea
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Abstract
The present invention provides a modified Bacillus cell for the production of a compound of interest, wherein the Bacillus cell comprises a genetic modification that leads to decreased production of enzymes with lipase and/or esterase activity. The use of compounds of interest produced with said improved Bacillus host cells reduces the formation of malodour and/or off-flavour when used for applications such as washing or cleaning or as feed or food additives.
Claims14
A recombinant Bacillus cell comprising an inactivation of the yitV and ypmR gene.
A method for producing a compound of interest, comprising the steps of
- a) providing a modified Bacillus host cell comprising an inactivation of the yitV and ypmR gene,
- b) introducing into said modified Bacillus host cell an expression cassette for a compound of interest,
- c) cultivating the host cell under conditions which allow for the expression of the compound of interest, and
- d) optionally isolating the compound of interest from the cultivation medium.
A method for reducing the lipase and/or esterase activity in a Bacillus cell comprising inactivating the yitV and/or ypmR gene in the Bacillus cell.
A method for reducing the lipase and/or esterase activity of a compound of interest produced by a Bacillus cell comprising the steps of
- a) providing a modified Bacillus host cell comprising an inactivation of the yitV and/or ypmR gene,
- b) introducing into said modified Bacillus host cell an expression cassette for a compound of interest,
- c) cultivating the host cell under conditions which allow for the expression of the compound of interest, and
- d) optionally isolating the compound of interest from the cultivation medium.
A method for reducing the formation of malodour or off-flavor in a composition comprising a compound of interest produced by a Bacillus cell comprising the steps of
- a) providing a modified Bacillus host cell comprising an inactivation of the yitV and/or ypmR gene,
- b) introducing into said modified Bacillus host cell an expression cassette for a compound of interest,
- c) cultivating the host cell under conditions which allow for the expression of the compound of interest, and
- d) optionally isolating the compound of interest from the cultivation medium.
Dependencies are inferred from the claim text.