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US 2024/0052413 A1Published application
Methods of analyte detection
AssigneePacific Biosciences of California, Inc.
InventorsBryan P. STAKER, Niandong LIU, Michael David McLAUGHLIN, Bart Lee STAKER
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Abstract
Disclosed herein are methods and systems for detection and discrimination of optical signals from a densely packed substrate. These have broad applications for biomolecule detection near or below the diffraction limit of optical systems, including in improving the efficiency and accuracy of polynucleotide sequencing applications.
Claims11
-20. (canceled)
A method of identifying an analyte, comprising:
- (a) contacting a probe of a plurality of probes bound to a target analyte of a plurality of analytes with a reaction mixture comprising reagents and under conditions that result in synthesis of a polynucleotide strand using a tail region of said probe as a template, wherein a plurality of hydrogen ions is released upon synthesis of said polynucleotide strand;
- and
- (b) detecting said plurality of hydrogen ions using an electrical detector, thereby identifying said analyte of said plurality of analytes.
A method of identifying an analyte, comprising:
- (a) providing at least one analyte of a plurality of analytes immobilized on a substrate;
- (b) performing a plurality of cycles of probe binding to said at least one analyte, wherein said plurality of cycles of probe binding generates a sequence of optical signals;
- (c) detecting said sequence of optical signals with an imaging system;
- and
- (d) processing said sequence of optical signals with an expected sequence of optical signals corresponding to said analyte to generate a decoded sequence of optical signals identifying said analyte with an error rate less than 5% over at least 20 cycles of said plurality of cycles.
2 more elements
Dependencies are inferred from the claim text.