US 2024/0052387 A1 · VARIANTS OF GALA REDUCTASE AND THEIR USES

Abstract

The present invention relates to polypeptides which are galacturonate (GalA) reductase variants comprising at least one amino acid substitution at a position corresponding to K261 and/or R267. The present invention further relates to nucleic acid molecules encoding the polypeptides and to host cells containing said nucleic acid molecules. The present invention further relates to a method for the production of L-galactonate (GalOA) and/or other bio-based compounds, comprising the expression of said nucleic acid molecules, preferably in said host cells. The present invention also relates to the use of the polypeptides, nucleic acids molecule or host cells for the production of L-galactonate (GalOA) and/or other bio-based compounds, and/or for the recombinant fermentation of biomaterial containing D-galacturonate (GalA).

Claims21

Dependent claims

A polypeptide comprising an amino acid substitution at a position corresponding to K261 and/or R267 of the amino acid sequence of SEQ ID NO:

1, wherein the polypeptide has at least 80% sequence identity with the amino acid sequence of SEQ ID NO:
1.

Dependencies are inferred from the claim text.

Facts

Application

17/766,552Filed 8 Oct 2020

Priority

8 Oct 2019

Published

15 Feb 2024

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

C12N 15/52
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: genes encoding for enzymes or proenzymes
C12P 17/06
Oxygen as only ring hetero atoms: containing a six-membered hetero ring, e.g. fluorescein
C12N 9/0006
Oxidoreductases: acting on CH-OH groups as donors
C12N 15/81
For fungi: for yeasts

Variants of gala reductase and their uses

Life of this patent

Variants of gala reductase and their uses

Life of this patent

Abstract

Claims21