Protein having l-proline efflux function, and use thereof

A strain for producing L-proline, wherein the strain expresses the following polypeptide:

• A) a polypeptide having an amino acid sequence set forth in SEQ ID NO:
• 1 or SEQ ID NO:
• 2;
• or
• B) a polypeptide having some amino acids added or deleted at either or both ends of the polypeptide of A) but still having the activity of an L-proline efflux function;
• or
• C) a polypeptide having higher than 90%, 95%, 96%, 97%, 98%, or 99% homology to the polypeptide of A) and derived from Corynebacterium with the activity of an L-proline efflux function;
• preferably, the polypeptide has an amino acid sequence as set forth in SEQ ID NO:
• 1 or SEQ ID NO:
• 2;
• preferably, the strain is a bacterium;
• preferably, the strain is selected from Escherichia and Corynebacterium, preferably Escherichia coli and Corynebacterium glutamicum, more preferably Corynebacterium glutamicum, and more particularly Corynebacterium glutamicum ATCC 13032, Corynebacterium glutamicum ATCC 13869, Corynebacterium glutamicum B253, and Corynebacterium glutamicum ATCC 14067;
• more preferably, glutamate kinase in the bacterium is not subjected to feedback inhibition by L-proline or is attenuated feedback inhibition by L-proline;
• even more preferably, the activity of glutamate kinase and/or glutamate-semialdehyde dehydrogenase and/or pyrroline-5-carboxylate reductase in the bacterium is enhanced.

A host cell for producing hydroxyproline, wherein the activity of a polypeptide having an L-proline efflux function in the host cell is attenuated, wherein the polypeptide having the activity of the L-proline efflux function is:

• A) a polypeptide having an amino acid sequence set forth in SEQ ID NO:
• 1 or SEQ ID NO:
• 2;
• or
• B) a polypeptide having some amino acids added or deleted at either or both ends of the polypeptide of A) but still having the activity of the L-proline efflux function;
• or
• C) a polypeptide having higher than 90%, 95%, 96%, 97%, 98%, or 99% homology to the polypeptide of A) and derived from Corynebacterium with the activity of an L-proline efflux function;
• preferably, the host cell is selected from Escherichia and Corynebacterium, preferably Escherichia coli and Corynebacterium glutamicum, more preferably Corynebacterium glutamicum, and more particularly Corynebacterium glutamicum ATCC 13032, Corynebacterium glutamicum ATCC13869, Corynebacterium glutamicum B253, and Corynebacterium glutamicum ATCC 14067;
• more preferably, glutamate kinase in the host cell is not subjected to feedback inhibition by L-proline or is subjected to an attenuated feedback inhibition by L-proline;
• even more preferably, the activity of a proline hydroxylase in the host cell is enhanced;
• even more preferably, the activity of glutamate kinase and/or glutamate-semialdehyde dehydrogenase and/or pyrroline-5-carboxylate reductase in the host cell is enhanced.