Process control for 3-hydroxypropionic acid production by engineered strains of aspergillus niger
AssigneeBattelle Memorial Institute
InventorsKyle R. Pomraning, Ziyu Dai, Jon K. Magnuson, Beth A. Hofstad, Jeffrey J. Czajka, Joonhoon Kim, Shuang Deng
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Abstract
Provided herein are recombinant Aspergillus niger capable of producing 3-hydroxypropionic acid (3-HP). Also provided are methods of producing 3-hydroxypropionic acid (3-HP) and related kits.
Claims21
A recombinant Aspergillus niger capable of producing 3-hydroxypropionic acid (3-HP), comprising an exogenous nucleic acid molecule encoding aspartate 1-decarboxylase (PAND), an exogenous nucleic acid molecule encoding β-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH).
A method of producing 3-HP, comprising:
- inoculating a media with Aspergillus niger comprising a 3-HP β-alanine pathway, thereby generating a cultured media, and
- fermenting the cultured media at a temperature of 30° C. to 37° C., under acidic and microaerobic conditions.
Dependencies are inferred from the claim text.