PREPARATION METHOD OF MOFs-COATED CARDIOMYOCYTE CORE-SHELL STRUCTURE

A preparation method of an MOFs-coated cardiomyocyte core-shell structure, comprising the following steps:

• step 1, discarding a supernatant of an HL-1 mouse cardiomyocyte culture medium, and cleaning HL-1 mouse cardiomyocytes with phosphate buffered saline (PBS) or normal saline;
• step 2, adding cleaned HL-1 mouse cardiomyocytes into a cell culture flask or a Petri dish, and adding 0.25% (w/w) trypsin in PBS, so that the 0.25% (w/w) trypsin in PBS is covered over the HL-1 mouse cardiomyocytes;
• step 3, putting the cell culture flask or the Petri dish in step 2 into a 37° C. CO2 incubator for digestion, and adding a culture medium to terminate the digestion to obtain a cell suspension;
• step 4, subjecting the cell suspension to centrifugation, and discarding a supernatant to obtain cell pellets;
• step 5, resuspending the cell pellets in a fresh culture medium, adding the cell pellets into a cell culture flask or a Petri dish, and culturing the cell culture flask or the Petri dish in the 37° C. CO2 incubator for 1-7 days to obtain suspension cells;
• step 6, subjecting the suspension cells obtained in step 5 to centrifugation, and collecting cell pellets;
• resuspending the cell pellets in a fresh culture medium, and transferring the cell pellets to a new cell culture flask or a Petri dish for passage, wherein the passage is conducted in a ratio of 1:(2-8);
• step 7, preparing a Zn(NO3)2·6H2O aqueous solution and a 2-methylimidazole aqueous solution, wherein
• the Zn(NO3)2·6H2O aqueous solution has a concentration of 1-10 g/L;
• and
• the 2-methylimidazole aqueous solution has concentration of 5-20 g/L;
• and
• step 8, separately adding the Zn(NO3)2·6H2O aqueous solution and the 2-methylimidazole aqueous solution to the cell culture flask or the Petri dish obtained in step 6, and transferring the cell culture flask or the Petri dish to the 37° C. CO2 incubator for culture for 1-7 days to obtain the MOFs-coated cardiomyocyte core-shell structure.