Methods for culturing methanotrophic bacteria and isolating proteins from bacterial biomass
AssigneeCalysta, Inc.
InventorsYelena Stegantseva, Lisa Marie Newman, Warren Kwan, Chien-Seng Hwang, Lorraine Joan Giver, Celine Schiff-Deb
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Abstract
The present disclosure provides method for generating biomass by culturing methanotrophic bacteria under low copper conditions. Also provided are generated biomass and protein isolate prepared from the biomass.
Claims26
A method for generating biomass, comprising:
- (a) continuously culturing a methanotrophic bacterium at a copper level no more than 100 mg copper per kg dry cell weight (DCW) to generate biomass.
A bacterial biomass comprising primarily, consisting essentially of, or consisting of, a biomass of a methanotrophic bacterium having a copper level no more than 100 mg copper per kg dry cell weight (DCW).
A protein isolate prepared from a bacterial biomass that comprises primarily a biomass of a methanotrophic bacterium, wherein the protein isolate comprises at least 82% crude protein, preferably at least 85% crude protein.
Dependencies are inferred from the claim text.