Synthesis of peg-based thiol-norbornene hydrogels with tunable hydroylitic degradation properties
AssigneeThe Trustees of Indiana University
InventorsChien-Chi Lin, Fang-Yi Lin
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Abstract
Methods for synthesizing a hydrogel having tunable hydrolylic degradation kinetics according to certain embodiments include synthesizing a norborene-functionalized polyethylene glycol macromer having an ester linkage between a polyethylene glycol domain of the macromer and a norbornene domain of the macromer and having a carboxyl group on the norbornene; and reacting a quantity of such macromers with a quantity of dithiol molecules by an ultraviolet-initiated photo-gelation reaction to yield a hydrogel. Such macromers and such hydrogels form additional aspects of the disclosure, as do a wide variety of methods for tuning and for using such hydrogels.
Claims42
A method for synthesizing a norbornene-functionalized polyethylene glycol macromer, comprising:
- reacting a polyethylene glycol reactant, the polyethylene glycol reactant comprising at least one polyethylene glycol domain with a terminal hydroxyl group, with carbic anhydride in the presence of a nucleophilic catalyst to yield a norbornene-functionalized polyethylene glycol macromer having an ester linkage between a polyethylene glycol domain of the macromer and a norbornene domain of the macromer, wherein the norbornene domain comprises a carboxyl group.
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A macromer for producing a hydrogel, the macromer comprising a multi-arm polyethylene glycol core having at least two polyethylene glycol domains, each polyethylene glycol domain connected through an ester linkage to a norbornene moiety, wherein at least one norbornene moiety of the macromer comprises a carboxyl group.
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A macromer for producing a hydrogel, the macromer comprising a multi-arm polyethylene glycol core having at least two polyethylene glycol domains, each polyethylene glycol domain connected through an ester linkage to a norbornene moiety, wherein at least one norbornene moiety of the macromer is connected through a carboxamide linkage to a functional group.
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A method for forming a macroporous hydrogel, the macroporous hydrogel comprising at least one cell, the method comprising:
- mixing a hydrogel-forming solution with a media containing the at least one cell, the hydrogel-forming solution comprising a crosslinkable material;
- crosslinking at least a portion of the crosslinkable material of the hydrogel-forming solution, thereby forming a microgel comprising the at least one cell and a sacrificial material;
- dispersing the microgel in a bulk hydrogel;
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- removing the sacrificial material, thereby forming the macroporous hydrogel.
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Dependencies are inferred from the claim text.