Pcr detection chip, associated test device and implementation analysis system
AssigneeBforcure
InventorsMaël LE BERRE, Claire LEBOUTEILLER, Bilal HADJI
Life of this patent
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Abstract
A system for testing a microfluidic sample chip to perform PCR and/or fluorescence type testing of the biological samples. The chip having the form of a hollow block including at least one chamber delimited by an upper wall and a lower wall. The chamber receiving a sample to be tested and the chamber being associated with thermalization element and with a sensor to measure the fluorescence. The system further includes a tab, preferably opaque and/or rigid or semirigid, positioned in the continuation of, and preferably in the same plane as, the walls.
Claims19
1-11. (canceled)
A system to test a microfluidic sample chip to perform a polymerase chain reaction (PCR) type testing of biological samples, the chip having a shape of a block, said chip comprising at least one hollow chamber to receive a sample, delimited by a first wall made out of a high thermal conductivity material, a material with a thermal conductivity greater than 15 Wm−1K−1, and a second wall made at least partially out of a transparent material, the block comprising at least two first openings to allow introduction of the sample into said at least one hollow chamber and evacuation of the atmosphere in said at least one hollow chamber during the introduction of the sample, the first wall and the second wall being parallel to each other, the chip comprising a tab arranged in an extension of the first and second walls, and a sealing device, arranged in contact with the second wall, the system comprising:
- a side wall provided with a second opening to allow a passage of the chip towards a thermalization element;
- a complementary polarizing element configured to cooperate with a polarizing element on the chip;
- a transparent element configured to maintain the chip in contact with the thermalization element after introduction the chip through the second opening, the contact between the chip and the thermalization element being generated by a cooperation of the polarizing element on the chip and the complementary polarizing element;
- and
- a sensor to measure fluorescence of the sample.
Dependencies are inferred from the claim text.