Methods and devices for ultrasensitive direct detection of microorganisms

A composition consisting essentially of:

• a magnesium salt;
• a buffering agent;
• water;
• and
• a compound of Formula 1:
• wherein R1 is selected from the group consisting of optionally substituted, branched or unbranched, saturated or unsaturated C1-C8 aliphatic;
• optionally substituted, saturated or unsaturated C3-C14 carbocyclic;
• optionally substituted, saturated or unsaturated 3-8 membered heterocyclic;
• optionally substituted, branched or unbranched, saturated or unsaturated ((Ra)q—(C═O)—(Ra)q)p;
• optionally substituted C6-C14 aryl;
• and optionally substituted 3-8 membered heteroaryl;
• and/or any suitable combinations thereof;
• wherein R2 is selected from the group consisting of hydrogen;
• optionally substituted, branched or unbranched, saturated or unsaturated C1-C28 aliphatic;
• optionally substituted, branched or unbranched, saturated or unsaturated —(Rb—(O—Rb)n—O—Rb)p;
• optionally substituted, branched or unbranched, saturated or unsaturated —(Rb—(O—Rb)n—NH—Rb)p;
• optionally substituted, branched or unbranched, saturated or unsaturated —(Rb—(O—Rb—O)n—S—Rb)p;
• optionally substituted, branched or unbranched, saturated or unsaturated —(Rb—(S—Rb)n—S—Rb)p;
• optionally substituted C6-C14 aryl;
• optionally substituted 3-8 membered heteroaryl;
• optionally substituted, saturated or unsaturated C3-C14 carbocyclic;
• optionally substituted, saturated or unsaturated 3-8 membered heterocyclic;
• optionally substituted, branched or unbranched, saturated or unsaturated —(C═O)—(Rb);
• optionally substituted, branched or unbranched, saturated or unsaturated —((Ra)q—O—(Ra)q)p—;
• optionally substituted, branched or unbranched, saturated or unsaturated —((Ra)q—NH—(Ra)q)p—;
• optionally substituted, branched or unbranched, saturated or unsaturated —((Ra)q—N(Ra)—(Ra)q)p—;
• and optionally substituted, branched or unbranched, saturated or unsaturated —((Ra)q—S—(Ra)q)p—;
• and/or any suitable combinations thereof;
• wherein each occurrence of R a is independently C1-C8 aliphatic or C6-C14 aryl;
• wherein each occurrence of Rb is independently C1-C15 aliphatic or C6-C14 aryl;
• wherein each occurrence of subscript q is independently an integer between 0 and 1, inclusive;
• wherein each occurrence of subscript p is independently an integer between 1 and 6, inclusive;
• and
• wherein each occurrence of subscript n is independently an integer between 0 and 14, inclusive.