Back to results
US 2024/0018480 A1Published application
Enhancement of extracellular vesicle production by lysosome inhibitor
AssigneeExoTop Theragnostics Inc.
InventorDongki KIM
Life of this patent
Estimating the term…
Abstract
Provided are methods and systems for an enriched production of high-quality extracellular vesicles (EVs) from a mammalian cell. In some cases, the methods may comprise culturing the cell in a chemically-defined protein-free (CDPF) medium with the addition of a lysosome inhibitor to increase production of EVs. In some cases, the CDPF medium is supplemented with additives.
Claims28
-26. (canceled)
A method for increasing production of extracellular vesicles (EVs), the method comprising:
- a. culturing mammalian cells in a chemically-defined, protein-free (CDPF) medium and a lysosome inhibitor to produce a conditioned culture medium, wherein the lysosome inhibitor is chloroquine, wherein a concentration of chloroquine is about 50 μM to about 200 μM;
- b. separating the conditioned culture medium after cell culture from the cells;
- and
- c. purifying EVs from the conditioned culture medium.
1 more element
(canceled)
Dependencies are inferred from the claim text.