Flavone 4'-o-methyltransferase gene and use for same
AssigneeSuntory Holdings Limited
InventorNoriko Nakamura
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Abstract
Transgenic plants with modified flower color, or their inbred or outbred progeny, or their propagates, partial plant bodies, tissues or cells, are provided. A delphinidin-type anthocyanin and a flavone C-glycoside in which the 7-position and T-position hydroxyl groups are methylated, are caused to coexist in plant cells.
Claims26
A polynucleotide selected from the group consisting of the following (A) to (E):
- (A) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:
- 19 or SEQ ID NO:
- 21;
- (B) a polynucleotide that hybridizes with a polynucleotide comprising the nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO:
- 19 or SEQ ID NO:
- 21 under stringent conditions, and that encodes a protein having activity of transferring a methyl group to the 4′-position hydroxyl group of flavone C-glycoside;
- (C) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO:
- 20;
- (D) a polynucleotide encoding a protein comprising an amino acid sequence that is the amino acid sequence of SEQ ID NO:
- 20 having a deletion, substitution, insertion and/or addition of one or more amino acids, and having activity of transferring a methyl group to the 4′-position hydroxyl group of flavone C-glycoside;
- and
- (E) a polynucleotide encoding a protein comprising an amino acid sequence with at least 90% identity with respect to the amino acid sequence of SEQ ID NO:
- 20 and having activity of transferring a methyl group to the 4′-position hydroxyl group of flavone C-glycoside.
A method for creating a transgenic plant with modified flower color, wherein the method comprises a step of causing a delphinidin-type anthocyanin and a flavone C-glycoside to coexist in plant cells, where the 7-position and 4′-position hydroxyl groups of flavone C-glycoside are methylated.
The method according to claim 25, wherein the plant is a rose, petunia, chrysanthemum, carnation or lily.
Dependencies are inferred from the claim text.