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US 2024/0010997 A1Published application
Cytochrome p450 enzyme mutant and application thereof
AssigneeAsymchem Life Science (Tianjin) Co., Ltd
InventorsHao HONG, Gage JAMES, Na ZHANG, Xuecheng JIAO, Yulei MA, Shan CAO, Yibing CHENG, Kaihua SUN
Life of this patent
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Abstract
Provided are a cytochrome P450 enzyme mutant and an application thereof. The enzyme activity and anti-Markov oxidation selectivity of P450 derived from a wild-type strain of Bacillus megaterium undergo protein modification by means of directed evolution, thus improving enzyme activity and selectivity, and developing a series of P450 enzyme mutants that may be used for industrial production.
Claims15
A cytochrome P450 enzyme mutant, having an amino acid sequence of SEQ ID NO:
- 1 with a mutation of one or more amino acids:
- V79A + F332A
- V79A + F332A + A75V
- V79A + F332A + A75F
- V79A + F332A + L76A
- V79A + F332A + A83F
- V79A + F332A + A83V
- V79A + F332A + F88A
- V79A + F332A + F88V
- V79A + F332A + T89A
- V79A + F332A + T89V
- V79A + F332A + A265V
- V79A + F332A + A265F
- V79A + F332A + T269V
- V79A + F332A + T269A
- V79A + F332A + T269F
- V79A + F332A + A329V
- V79A + F332A + A329F
- V79A + F332A + A331F
- V79A + F332A + A331V
- V79A + F332A + L438A
- V79A + F332A + L438F
- V79A + F332A + C63Y
- V79A + F332A + C63Y + S107C
- V79A + F332A + C63Y + S107C + Q129L
- V79A + F332A + C63Y + S107C + Q129L + E229K
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + N320I
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + S165G
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A331P
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A331G
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A331D
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329L
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A75R
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329Y + A331P
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329L + A331P
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + A331F
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + A331R
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + A331D
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + R48H
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + Y52G
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + Q74L
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + T269P
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333H
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333M
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + M355F
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + M355K
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + L182I
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + L182M
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + A331F + S333Q
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + A331F + S333V
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + A331F + W91M
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + A331F + S90C
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + A331F + L18V
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + A331F + S90V
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + A331F + S90G
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333H + L182A
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333H + L182G
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333H + L182M
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333H + L182F
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333H + A331P
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333H + A331S
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333M + M355W
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333M + M355G
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333M + A331D
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333H + L182F + S73G
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333H + L182F + W91F
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333H + L182F + P173Q
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333H + L182F + P173R
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333H + L182F + L72F
- V79A + F332A + C63Y + S107C + Q129L + E229K + Q388L + T428I + F78L + I175S + A329K + S333H + L182F + S73A
72 more elements
Dependencies are inferred from the claim text.