Production of lytic phages

A method for producing lytic phage particles or lytic phage-derived delivery vehicles, comprising:

• (a) providing a production bacterial cell stably comprising phage structural genes and phage DNA packaging genes of a lytic bacteriophage,
• wherein the expression of said phage structural genes and phage DNA packaging genes in said production bacterial cell is controlled by an induction mechanism comprising phage excision/insertion genes, phage DNA replication genes, and phage regulation genes of a second, non-lytic bacteriophage, wherein said phage excision/insertion genes, phage DNA replication genes and phage regulation genes are neither phage DNA packaging genes nor phage structural genes,
• wherein said production bacterial cell does not comprise phage excision/insertion genes and/or phage replication genes of the lytic bacteriophage,
• wherein said production bacterial cell is a P. freudenreichii bacterial cell,
• wherein the lytic bacteriophage is a C. acnes phage and
• wherein the second, non-lytic bacteriophage is a P. freudenreichii phage;
• and
• (b) inducing, in said production bacterial cell, expression of said phage structural genes and phage DNA packaging genes, and assembly of the products expressed by said phage structural genes and phage DNA packaging genes, thereby producing lytic phage particles or lytic phage-derived delivery vehicles.