US 2024/0010982 A1 · METHOD FOR PRODUCING CARTILAGE CELLS INDUCED TO BE DIFFERENTIATED FROM STEM CELLS

Abstract

The present invention relates to a method for inducing differentiation, into chondrocytes, of cord blood mononuclear cell-derived induced pluripotent stem cells. In a case where a chondrogenic pellet produced by the method of the present invention is transplanted into a cartilage damage area in vivo, regeneration of cartilage can be effectively exhibited by differentiated chondrocytes. In such a case, an effective cartilage regeneration capacity can be exhibited as compared with a case where chondrocytes produced by differentiation induction with the addition of a recombinant growth factor are transplanted. Thus, the present invention can be usefully used for tissue engineering therapies.

Claims10

Dependent claims

A method for producing chondrocytes obtained by differentiation induction from stem cells, comprising:

i) culturing induced pluripotent stem cells (iPSCs) to generate embryoid bodies (EBs);
ii) culturing the EBs generated in step i) in a gelatin-coated medium, to obtain outgrowth cells (OG cells);
iii) transducing the OG cells obtained in step ii) with either or both of a minicircle vector that contains a base sequence encoding BMP2 and a minicircle vector that contains a base sequence encoding TGFβ3;
iv) inducing differentiation of the OG cells transduced in step iii) into chondrocytes;
and
v) obtaining the chondrocytes produced by differentiation induction in step iv).

3 more elements

Dependencies are inferred from the claim text.

Method for producing cartilage cells induced to be differentiated from stem cells

Life of this patent

Method for producing cartilage cells induced to be differentiated from stem cells

Life of this patent

Abstract

Claims10