Method for synthesizing labeled pyruvic acid
AssigneeShenzhen Zhonghe Headway Bio-Sci & Tech Co., Ltd.
InventorsJing QING, Xuelei JIA, Kai CHEN, Guowei LI, Quansheng HAN, Desheng DAI
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Abstract
A method for synthesizing a labeled pyruvic acid is provided. An isopropenyl Grignard reagent is added to a reaction vessel and cooled to a preset temperature. A carbon isotope-labeled carbon dioxide is added to the reaction vessel and continuously reacted with the isopropenyl Grignard reagent for a preset time. The reaction system is added with an acid and adjusted to a preset temperature to quench the reaction. The reaction system is subjected to ozonization, and reduced in the presence of a reducing agent. Impurities are removed to obtain the labeled pyruvic acid.
Claims10
A method for synthesizing a labeled pyruvic acid, comprising:
- (S1) adding an isopropenyl Grignard reagent to a reaction vessel followed by cooling to a preset temperature;
- (S2) adding a carbon isotope-labeled carbon dioxide to the reaction vessel followed by reaction with the isopropenyl Grignard reagent for a preset time;
- (S3) adding an acid to a reaction system obtained in step (S2) and adjusting the reaction system to a preset temperature to quench the reaction;
- (S4) subjecting a quenched reaction system to ozonization to obtain an ozonized product;
- (S5) reducing the ozonized product obtained in step (S4) with a reducing agent to obtain a reduced product;
- and
- (S6) removing impurities from the reduced product to obtain the labeled pyruvic acid.
Dependencies are inferred from the claim text.