Quantitative phase image generating method, quantitative phase image generating device, and program
AssigneeNikon Corporation
InventorsShota TSUCHIDA, Satoshi IKEDA
Life of this patent
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Abstract
A quantitative phase image generating method for a microscope, includes: irradiating an object with illumination light; disposing a focal point of an objective lens at each of a plurality of positions that are mutually separated by gaps Δz along an optical axis of the objective lens, and detecting light from the object; generating sets of light intensity distribution data corresponding to each of the plurality of positions based upon the detected light; and generating a quantitative phase image based upon the light intensity distribution data; wherein the gap Δz is set based upon setting information of the microscope.
Claims7
A quantitative phase image generating device for a microscope, the device comprising:
- a light source that irradiates illumination light to an object;
- a first detection unit that detects a first observation light, which is fluorescence from the object irradiated by the illumination light, via a first optical system;
- a first image generation unit that generates a fluorescence observation image based on the detected first observation light;
- a second detection unit that detects, with plural different conditions, a second observation light, which is light transmitted from the object irradiated by the same illumination light from the light source, via a second optical system, the second observation light being different from the first observation light;
- and
- a second image generation unit that generates a quantitative phase image based on the detected second observation light.
A quantitative phase image generating device comprising:
- a processor;
- and
- a memory encoded with instructions executed by the processor, the instructions causing the processor to perform operations comprising:
- causing an illumination light source to irradiate illumination light to an object;
- causing to be detected a first observation light, which is fluorescence from the object irradiated by the illumination light via a first optical system;
- generating a fluorescence observation image based on the detected first observation light;
- causing to be detected, with plural different conditions, a second observation light, which is light transmitted from the object irradiated by the same illumination light from the light source, via a second optical system, the second observation light being different from the first observation light;
- and
- generating a quantitative phase image based on the detected second observation light.
Dependencies are inferred from the claim text.