US 2024/0002943 A1Published application

A method for classifying the gut inflammation status in avian species

AssigneeEvonik Operations GmbH

InventorsFlorian BÖHL, Frank LYKO, Walter PFEFFERLE, Andreas KAPPEL, Rose WHELAN, Günter RADDATZ, Monika FLÜGEL, Stefan PELZER, Achim MARX, Emery STEPHANS, Frank THIEMANN, Emeka Ignatius IGWE

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Abstract

The invention provides a method for classifying the gut inflammation status of an avian subject or of a group of avian subjects to be tested, the method comprising the comparison of the average methylation levels within a panel of pre-selected LMRs in the genomic DNA isolated from gut sample material deriving from the individual avian subject or of the group of avian subjects to be tested with the average methylation levels of the same panel of LMRs in the genomic DNA pertaining to one or more reference samples having a negative gut inflammation status.

Claims15

Dependent claims

A method for classifying gut inflammation status of an avian subject or of a group of avian subjects to be tested, an avian test subject, the method comprising:

a. determining a test average methylation level of a panel of pre-selected Low-Methylated Regions (LMRs) in isolated genomic DNA from a gut test sample derived from the avian test subject;
b. comparing the test average methylation level obtained in step (a) with a reference average methylation level of the same panel of LMRs in genomic DNA isolated from at least one avian gut sample having a negative gut inflammation status,
wherein when the test average methylation level is substantially similar to the reference average methylation level, then the test subject has a negative gut inflammation status and when the test average methylation level is different from the reference average methylation level then the test subject has a positive gut inflammation status.

A method for developing a test system for classifying gut inflammation status of avian gut samples, the method comprising

a. detecting Low-Methylated Regions (LMRs) in genomic DNA in gut sample obtained from an avian subject or from a group of avian subjects with a known gut inflammation status,
b. selecting a panel of LMRs from the LMRs of step (a) suitable for each known gut inflammation status, such that the classification reliability of the selected panel of LMRs is optimized for the assignment of the known gut inflammation status,
c. measuring the average methylation levels of the selected panel of LMRs for each known gut inflammation status, and
d. creating a library of different reference average methylation levels of the selected panel of LMRs for respective known gut inflammation status,
wherein a comparison of an average methylation level obtained from a gut test sample with the reference average methylation levels of the selected panel of LMRs allows for classifying the gut inflammation status of the gut test sample.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application: 18/265,029Filed 22 Nov 2021
Priority: EP 20211764.44 Dec 2020
Published: 4 Jan 2024
Term ends: Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12Q 1/6883
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for diseases caused by alterations of genetic material
G16B 40/00
Ict specially adapted for biostatistics
C12Q 2523/125
Characterised by chemical treatment: bisulfite
C12Q 2539/107
The purpose being sequence identification by analysis of gene expression or genome comparison characterised by: representational Difference…