Compositions and methods for making (r)-reticuline and precursors thereof
AssigneeAntheia, Inc.
InventorsPeter James Facchini, Scott Cameron Farrow, Guillaume Arthur Welch Beaudoin
Life of this patent
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Abstract
Methods that may be used for the manufacture of the chemical compound (R)-Reticuline and synthesis precursors thereof. Compositions useful for the synthesis (R)-Reticuline and synthesis precursors are also provided.
Claims18
1-84. (canceled)
A recombinant nucleic acid comprising a nucleic acid sequence at least 70% identical to one or more of the nucleic acid sequences selected from the group consisting of:
- (i) SEQ ID NO:
- 116-218, SEQ ID NO:
- 324, and SEQ ID NO:
- 337;
- (ii) a nucleic acid sequence encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 219-321, SEQ ID NO:
- 325, and SEQ ID NO:
- 338;
- (iii) SEQ ID NO:
- 1-58, SEQ ID NO:
- 326, SEQ ID NO:
- 328, and SEQ ID NO:
- 339;
- (iv) a nucleic acid sequence encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 59-115, SEQ ID NO:
- 327;
- SEQ ID NO:
- 329, SEQ ID NO:
- 330, and SEQ ID NO:
- 340;
- (v) SEQ ID NO:
- 322;
- and
- (vi) a nucleic acid sequence encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 323.
A chimeric nucleic acid sequence comprising:
- (a) a first nucleic acid sequence encoding a CYP450 polypeptide;
- and
- (b) a second nucleic acid sequence encoding an AKR polypeptide;
- and
- (c) one or more nucleic acid sequences capable of controlling expression in a host cell.
A recombinant expression vector comprising as operably linked components:
- (a) a nucleic acid sequence capable of controlling expression in a host cell;
- and
- (b) a nucleic acid sequence encoding CYP450 and AKR;
- wherein the expression vector is suitable for expression in a host cell.
A host cell comprising a nucleic acid sequence according to claim 1.
Dependencies are inferred from the claim text.