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AssigneeNational University Corporation Kagawa University
InventorsKazuya AKIMITSU, Ken IZUMORI, Akihide YOSHIHARA, Shiro KATO, Susumu MOCHIZUKI, Hiromi YOSHIDA, Shigehiro KAMITORI
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A highly safe and highly heat-resistant L-rhamnose isomerase usable in the food industry and the production thereof are disclosed. The L-rhamnose isomerase is derived from a microorganism belonging to the genus Enterobacter, has a subunit molecular mass of 47 kDa as measured by SDS-PAGE, and has the following substrate specificities (A) isomerase activity that recognizes and reacts with a CHO group at C1 and an OH group at C2 of an aldose to convert the CHO group at C1 into an OH group and the OH group at C2 into a CO group, or recognizes and reacts with an OH group at C1 and a CO group at C2 of a ketose to convert the OH group at C1 into a CHO group and the CO group at C2 into an OH group; and (B) reactivity with L-rhamnose, L-lyxose, L-mannose, D-allose, D-ribose, and L-talose.
1-16. (canceled)
L-rhamnose isomerase derived from Enterobacter roggenkampii NrT7-1 internationally deposited at Patent Microorganisms Depositary under accession number of NITE BP-03309, having a subunit molecular mass of 47 kDa as measured by SDS-PAGE, and having the following substrate specificities (A) and (B) and the following physicochemical properties (C) and (D):
A protein described in the following (a) or (b):
A DNA described in the following (a) or (b):
A method of producing a D-allulose or D-allose, comprising causing a protein having L-rhamnose isomerase activity to a solution containing at least one selected from D-allose or D-allulose to produce a corresponding D-allulose or D-allose and collecting the resulting D-allulose or D-allose, wherein the protein comprises an amino acid sequence having 90% or more identity with an amino acid sequence represented by SEQ ID NO:
Dependencies are inferred from the claim text.