Methods and systems for evaluation of immune cell infiltrate in tumor samples

A method of evaluating a tumor, the method comprising:

• (a) quantitating on an image analysis system:
• (a1) a density of total lymphocytes within a region of interest (ROI) of a digital image of a tissue section of the tumor, wherein the ROI is selected from the group consisting of a tumor core (TC) region, an invasive margin (IM) region, and/or a peritumoral (PT) region of the tissue section;
• and
• (a2) a density of cells stained for at least one immune cell marker within a ROI of a digital image of a tissue section of the tumor, wherein:
• (a2a) the at least one immune cell marker is selected from the group consisting of CD8, CD4, FoxP3, CD45RA, and CD45RO;
• and
• (a2b) the ROI is selected from the group consisting of a TC region, an IM region, and/or a PT region of the tissue section;
• and
• (b) applying a non-linear continuous scoring function to a feature vector comprising the cell density of the lymphocytes and the density of cells staining for the at least one additional marker to obtain an immune context score (ICS) for the tumor, wherein the ICS is indicative of an outcome of a patient having the tumor.