System and method for microscope high-speed auto-focusing including an imaged subject based on corrected contrast distribution
AssigneeAraceli Biosciences Inc.
InventorShiou-jyh Ja
Life of this patent
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Abstract
Methods and systems are provided for microscope auto-focusing. In one example, a method for microscope auto-focusing comprises: acquiring a plurality of contrast samples of an imaged subject; modeling a contrast distribution based on the plurality of contrast samples; acquiring an additional contrast sample based on the contrast distribution; modeling a corrected contrast distribution based on the additional contrast sample and the plurality of contrast samples; and focusing the imaged subject based on the corrected contrast distribution.
Claims20
A method for microscope auto-focusing, comprising:
- measuring a focus of a subject in an initial position;
- moving the subject by a first amount from the initial position to a second position, and measuring the focus of the subject in the second position;
- moving the subject by a second amount from the second position to a third position, where the third position is based on a difference between the measured focus in the initial position and the measured focus in the second position, and measuring the focus of the subject in the third position;
- and
- moving the subject by a third amount from the third position to a fourth position, where the fourth position is determined via a modeled curve including the measured focus of the subject at the initial position, second position, and third position.
A method for auto-focusing a microscope, comprising:
- sequentially acquiring a first contrast measurement, a second contrast measurement, and a third contrast measurement of a subject, with the subject spaced apart from a microscope lens by a first amount, a second amount, and a third amount, respectively;
- and
- adjusting the spacing between the subject and the microscope lens to a fourth amount based on the first contrast measurement, the second contrast measurement, and the third contrast measurement, where the fourth amount of spacing between the subject and the microscope lens is within a threshold range of a spacing providing a peak focusing of the subject through the microscope lens.
A system, comprising:
- a microscope assembly including a microscope lens and a stage configured to support a subject to be imaged;
- and
- a controller with computer readable instructions stored on non-transitory memory that when executed, cause the controller to:
- sequentially acquire a first contrast measurement, a second contrast measurement, and a third contrast measurement of the subject, with the subject spaced apart from the microscope lens by a first amount, a second amount, and a third amount, respectively;
- and
- adjust the spacing between the subject and the microscope lens to a fourth amount based on the first contrast measurement, the second contrast measurement, and the third contrast measurement, where the fourth amount of spacing between the subject and the microscope lens is within a threshold range of a spacing providing a peak focusing of the subject through the microscope lens.
Dependencies are inferred from the claim text.