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US 2023/0417778 A1Published application
Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
AssigneePromega Corporation
InventorsCarolyn W. Hitko, Thomas Kirkland, Thomas Machleidt, Rachel Friedman Ohana, Matt Robers, Keith Wood
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Abstract
Provided herein are compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.
Claims19
A system comprising:
- (a) a bioactive agent conjugated to a fluorophore;
- (b) a polynucleotide for expression of a protein fusion in a cell, said polynucleotide comprising:
- (i) a protein of interest and (ii) a luciferase, wherein the bioactive agent is capable of binding non-covalently to the protein of interest upon interaction therewith, wherein the emission spectrum of the luciferase overlaps with the excitation spectrum of the fluorophore;
- and
- (c) a substrate for the luciferase.
2 more elements
The system of claim 3, wherein the nucleic acid is RNA.
Dependencies are inferred from the claim text.