Automated analysis of analytical gels and blots
AssigneeQuest Diagnostics Investments Incorporated
InventorsNilesh Saratkar, Stanley Naides, Michelle A. Cleveland
Life of this patent
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Abstract
The present solution describes an automated system for analyzing analytical gels or blots, such as electrophoresis gels. The system can automatically detect the lanes within the gel and convert the lane into a feature vector that can be compared to reference datasets. Based on a comparison of the feature vector to the reference datasets, the system can automatically classify the feature vector (and the test sample in the lane) into a phenotype group.
Claims21
-20.
A method of improved analytic gel or analytic blot analysis comprising:
- (i) obtaining a digital image of an analytic gel or analytic blot from an imager or an image database;
- (ii) identifying a plurality of lanes in the digital image;
- (iii) determining a lane intensity vector for each of the identified plurality of lanes;
- (iv) determining a correlation score between each of the lane intensity vectors and a reference lane intensity vector;
- and
- (v) classifying each of the identified plurality of lanes based on the correlation score.
A method of classifying a test sample to a phenotype comprising:
- (i) obtaining a digital image of an analytic gel or blot of one or more samples in a plurality of lanes from an imager or an image database;
- (ii) identifying the plurality of lanes in the digital image;
- (iii) determining a lane intensity vector for each of the identified plurality of lanes;
- (iv) determining a correlation score between each of the lane intensity vectors and a reference lane intensity vector;
- and
- (v) classifying a phenotype based in the correlation score to each of the identified plurality of lanes based on the correlation score.
Dependencies are inferred from the claim text.