Back to results
US 2023/0417672 A1Published application
Single copy level detection of coronaviruses
OwnerJeong-Yeol Yooninventor
Life of this patent
Estimating the term…
Abstract
A device for detecting and/or quantifying a coronavirus comprising a paper microfluidic chip, and the use thereof.
Claims24
A device for detecting and/or quantifying a coronavirus comprising a paper microfluidic chip, comprising one or more microfluidic channels, wherein said paper has a pore size of about 5 μm to 15 μm, and wherein said channels have a width about 2 mm to about 5 mm and/or a channel length between channel length of about 20 mm to about 50 mm.
A method for detecting a virus, said method comprising
- (a) applying a suspension comprising said virus to a paper microfluidic chip;
- (b) adding an anti-virus antibody conjugated fluorescent submicron particle suspension to the paper microfluidic chip;
- (c) allowing particles and viruses spread spontaneously throughout the paper microfluidic channel via capillary action, allowing the particles to aggregate and facilitating imaging of individual particles.
(canceled)
(canceled)
(canceled)
A kit for detecting a virus comprising
- a paper microfluidic chip with a pore size of about 5 μm to 15 μm depending on the type of sample suspension,
- a suspension of antibody conjugated fluorescent particles (wherein the volume of the antibody conjugated fluorescent particles is from 2 uL to 6 uL and a concentration from about 0.001% to about 0.04%, depending on the type of sample suspension), and optionally a smartphone-based fluorescence microscope.
(canceled)
Dependencies are inferred from the claim text.