Device and method for rapid determination of total bacterial count based on multi-wavelength reflectance spectrum

A device for rapid determination of total bacterial count based on multi-wavelength reflectance spectrum, comprising:

• a light-emitting element, configured to emit a continuous light beam;
• a monochromator, arranged in an emitting direction of the light beam and configured to separate the light beam into monochromatic lights with different wavelengths;
• an integrating sphere, configured to receive the monochromatic light separated by the monochromator and converge the diffusely reflected monochromatic light;
• a thermostatic element, arranged in the integrating sphere and configured to keep a standard sample at a constant temperature, wherein diffuse reflection occurs after the monochromatic light irradiates on a constant-temperature standard sample;
• a photoelectric conversion element, connected to the integrating sphere and configured to convert an optical signal into an electric signal;
• and
• an oscilloscope, connected to the photoelectric conversion element and configured to read, calculate and record the electric signal conducted by the photoelectric conversion element.

A method for rapid determination of total bacterial count based on multi-wavelength reflectance spectrum, comprising the following steps:

• sampling a to-be-tested sample, and preparing the to-be-tested sample into a standard sample;
• carrying out heating treatment on the standard sample, and then measuring a first average reflected light intensity value of the standard sample at different wavelengths under the irradiation of the monochromatic light with different wavelengths;
• after completing the first measurement, carrying out heating culture on the standard sample, and measuring a second average reflected light intensity value of the standard sample;
• calculating a difference value between the first average reflected light intensity value and the second average reflected light intensity value;
• and
• converting the difference value between the average reflected light intensity values into a value of the total bacterial count of the sample by means of a standard curve.