Screening platforms
AssigneeMayo Foundation for Medical Education and Research
InventorsMarina R. WALTHER-ANTONIO, Yuguang LIU, Samuel P. KOUNAVES, Dongyu LIU, Miles CLARK
Life of this patent
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Abstract
Materials and methods are provided herein for determining if a subject has, or is at risk of developing, a clinical condition (e.g., cervical cancer). For example, this document provides a cost-effective self-test for determining whether a biological fluid from a subject contains a high-risk HPV strain, and a method for use of the self-test.
Claims91
An elongate support comprising a first surface, a first nucleic acid immobilized on the first surface, and a second nucleic acid reversibly attached to the first surface or to a second surface, wherein the first surface is silanized, wherein the first nucleic acid is complementary to a first segment of a selected nucleic acid marker, and wherein the second nucleic acid is complementary to a second segment of the selected nucleic acid marker.
An elongate support comprising a silanized surface with a nucleic acid immobilized thereon, wherein the nucleic acid is complementary to a first segment of a selected nucleic acid marker.
A kit comprising:
- (a) an elongate support comprising a first surface, a first nucleic acid immobilized on the first surface, and a second nucleic acid reversibly attached to the first surface or to a second surface, wherein the first surface is silanized, wherein the first nucleic acid is complementary to a first segment of a selected nucleic acid marker, and wherein the second nucleic acid is complementary to a second segment of the selected nucleic acid marker and is labeled with a means for visual detection;
- (b) a receptacle for receiving a biological fluid sample;
- and
- (c) a vessel containing a substrate that interacts with the means for visual detection.
A kit comprising:
- (a) an elongate support comprising a silanized surface with a first nucleic acid immobilized thereon, wherein the first nucleic acid is complementary to a first segment of a selected nucleic acid marker;
- (b) a receptacle for receiving a biological fluid sample;
- (c) a first vessel containing a second nucleic acid that is complementary to a second segment of the selected nucleic acid marker, wherein the second nucleic acid is labeled with a means for visual detection;
- and
- (d) a second vessel containing a substrate that interacts with the means for visual detection.
A method for determining that a biological fluid contains a selected marker, wherein the method comprises:
- (a) providing an elongate support comprising a first surface, a first nucleic acid immobilized on the first surface, and a second nucleic acid reversibly attached to the first surface or to a second surface, wherein the first surface is silanized, wherein the first nucleic acid is complementary to a first segment of a selected nucleic acid marker, and wherein the second nucleic acid is complementary to a second segment of the selected nucleic acid marker and is labeled with a means for visual detection;
- (b) placing at least a portion of the elongate support into a vessel containing a biological fluid, such that the first or first and second surfaces are contacted by the biological fluid;
- (c) removing the elongate support from the vessel and rinsing the elongate support;
- (d) placing at least a portion of the elongate support into a vessel containing a substrate that interacts with the means for visual detection, such that the first surface is contacted by the substrate;
- and
- (e) visually inspecting the vessel containing the substrate, or a sample of the substrate, to determine that the signal is present, thus indicating the presence of the selected marker in the biological fluid.
A method for determining that a biological fluid contains a selected marker, wherein the method comprises:
- (a) providing an elongate support comprising a silanized surface with a first nucleic acid immobilized thereon, wherein the first nucleic acid is complementary to a first segment of a selected nucleic acid marker;
- (b) placing at least a portion of the elongate support into a first vessel containing a biological fluid, such that the silanized surface is contacted by the biological fluid;
- (c) removing the elongate support from the first vessel and rinsing the elongate support;
- (d) placing at least a portion of the elongate support into a second vessel comprising a second nucleic acid that is complementary to a second segment of the selected nucleic acid marker and is labeled with a means for visual detection, such that the silanized surface is contacted by the second nucleic acid;
- (e) removing the elongate support from the second vessel and rinsing the elongate support;
- (f) placing at least a portion of the elongate support into a third vessel containing a substrate that interacts with the means for visual detection, such that the silanized surface is contacted by the substrate;
- and
- (g) visually inspecting the third vessel containing the substrate, or a sample of the substrate, to determine that the signal is present, thus indicating the presence of the selected marker in the biological fluid.
A method for preparing a silanized glass surface, wherein the method comprises:
- reacting a glass surface with a silane-containing compound,
- coupling an amino group of the silane-containing compound to biotin-N-hydroxysuccinimide (biotin-NHS) to yield a silanized, biotinylated surface, and
- reacting the silanized, biotinylated surface with benzaldehyde.
Dependencies are inferred from the claim text.