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US 2023/0416832 A1Published application
Methods for dna methylation analysis
AssigneeThe General Hospital Corporation
InventorsA. John Iafrate, Ju Cheng
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Abstract
Next Generation Sequencing (NGS)-based methods using Methylation Sensitive Restriction Enzymes (MSREs) for methylation analysis of DNA, e.g., circulating tumor DNA.
Claims32
A method comprising:
- (a) providing a sample comprising DNA;
- (b) generating a first population of blunt-ended fragments from the DNA,
- (c) digesting the first population of fragments using one or more methylation sensitive restriction enzymes (MSREs), wherein the MSRE leaves a 5′-overhang of at least one nucleotide;
- (d) filling in the overhangs with modified nucleosides to create a second population of blunt-ended fragments;
- and
- (e) purifying fragments comprising modified nucleosides.
3 more elements
Dependencies are inferred from the claim text.