Target-enriched multiplexed parallel analysis for assessment of fetal dna samples

A kit comprising a container comprising a pool of TArget Capture Sequences (TACS), wherein the pool of TACS comprises a plurality of TACS families,

• wherein each TACS family comprises a plurality of member sequences,
• wherein each member sequence binds to the same genomic sequence of interest but has different start and/or stop positions with respect to a reference coordinate system for the genomic sequence of interest,
• and further wherein:
• (i) each member sequence within each TACS family is between 100-500 base pairs in length, each member sequence having a 5′ end and a 3′ end;
• (ii) each member sequence binds to the same genomic sequence of interest at least 50 base pairs away, on both the 5′ end and the 3′ end, from regions harboring Copy Number Variations (CNVs), Segmental duplications or repetitive DNA elements;
• and
• (iii) the GC content of the pool of TACS is between 19% and 80%, as determined by calculating the GC content of each member within each family of TACS.