Methods and compositions for loading of polymerase complexes
AssigneePacific Biosciences of California, Inc.
InventorErik Miller
Life of this patent
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Abstract
The present disclosure provides methods, compositions, and systems for distributing polymerase compositions into array regions. In particular, the described methods, compositions, and systems utilize density differentials and/or additives to increase efficiency in the distribution of polymerase compositions to a surface as compared to methods utilizing only diffusion control.
Claims15
1-87. (canceled)
A method of distributing polymerase compositions onto a surface, the method comprising:
- (a) providing a polymerase composition in a solution comprising a stabilizing additive;
- (b) adding the solution to the surface;
- and
- (c) dehydrating the solution to concentrate the polymerase composition to the surface,
- thereby distributing the polymerase composition to the surface.
The method of claim 111, wherein the carbohydrate additive comprises a member selected from the group consisting of sucrose, dextrose, trehalose, and Ficoll.
96-110. (canceled)
Dependencies are inferred from the claim text.