Method for labeling nucleic acid
AssigneeCambridge Enterprise Limited
InventorsManuel NAPPI, Alexandre HOFER, Shankar BALASUBRAMANIAN, Matthew J. GAUNT
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Abstract
The invention provides a method for labeling a nucleic acid comprising N6-methyl adenine. The method comprises forming an alpha-amino radical on the N6-methyl group of N6mAde, and capturing the alpha-amino radical with a radical acceptor comprising a nitrosopyridyl group. The presence of N6mAde in a nucleic acid may then be established by detection of the labeled nucleic acid, or the labeled nucleic acid may be extracted or further modified using the label. Also provided is a method for mapping the position of N6mAde within a target nucleic acid, and probe molecules and kits for use in the method.
Claims26
A method for labeling a nucleic acid comprising N6-methyl adenine (N6mAde), the method comprising:
- i) forming an alpha-amino radical on the N6-methyl group of N6mAde;
- and
- ii) capturing the alpha-amino radical with a radical acceptor comprising a nitrosopyridyl group (O═N-Py-).
-17. (canceled)
(canceled)
-55. (canceled)
A compound of formula (III):
- O2N-Py-L-C1 (III)
- where, -Py- is a pyridinediyl group, -L- is a linker and -C1 is a click reaction partner such as a C2-20 alkynyl group, such as an ethynyl group.
(canceled)
(canceled)
A kit comprising:
- (a) a compound comprising a nitropyridyl group (O2N-Py-);
- and
- (b) a tertiary amine;
- and
- (c) a photocatalyst,
-66. (canceled)
Dependencies are inferred from the claim text.