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US 2023/0416814 A1Published application
Nucleic acid sequencing-by-synthesis (sbs) methods that combine sbs cycle steps
AssigneeSingular Genomics Systems, Inc.
InventorsEli N. GLEZER, Andrew SPAVENTA, David BARKER
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Abstract
The present disclosure provides improved nucleic acid sequencing-by-synthesis (SBS) methods, related kits and reagents, and systems for performing such methods using such kits and reagents.
Claims21
1-33. (canceled)
A method of detecting a labeled nucleotide analogue, the method comprising:
- contacting a solid support comprising a primer hybridized to a template DNA molecule with a plurality of labeled nucleotide analogues;
- and binding a labeled nucleotide analogue to a complementary nucleotide of the template DNA molecule;
- removing unbound labeled nucleotide analogues;
- imaging said solid support and detecting the bound labeled nucleotide analogue;
- and, while imaging, contacting said solid support with a plurality of blocked nucleotide analogues, wherein each blocked nucleotide analogue comprises a blocking moiety.
2 more elements
Dependencies are inferred from the claim text.