US 2023/0416812 A1 · METHOD CAPABLE OF MAKING ONE CLUSTER BY CONNECTING INFORMATION OF STRANDS GENERATED DURING PCR PROCESS AND TRACKING GENERATION ORDER OF GENERATED STRANDS

Abstract

The present invention relates to a method capable of making one cluster by connecting information of strands generated during a PCR process and tracking the generation order of the generated strands. More specifically, the present invention uses a UID-containing primer so as to enable all parent strands and daughter strands to share one UID, and uses the shared UID so as to connect two strands (parent strand and daughter strand) and furthermore extend to and connect a granddaughter strand, thereby enabling connection to all progeny strands derived from a first copied strand. Accordingly, the present invention is capable of not only making one network (cluster), but also identifying the generation order of strands generated during an amplification process, constructing lineage of amplification, and observing error patterns.

Claims10

Dependent claims

A method for generating a consensus sequence for detecting a target nucleic acid, the method comprising:

amplifying DNA fragments from a sample using polymerase chain reaction (PCR) with primers containing adapter sequences, flanking sequences, and UID sequences, in the direction from the 5′ end to the 3′ end;
obtaining sequence information of the amplified DNA fragments through the PCR;
and
generating a cluster using a peer-to-peer (P2P) network method based on the obtained sequence information.

1 more element

Dependencies are inferred from the claim text.

Method capable of making one cluster by connecting information of strands generated during pcr process and tracking generation order of generated strands

Life of this patent

Method capable of making one cluster by connecting information of strands generated during pcr process and tracking generation order of generated strands

Life of this patent

Abstract

Claims10