METHOD FOR INDUCING AND DETECTING SOLUBLE LOX-1 (sLOX-1) IN CULTURED BLOOD CLOTS
AssigneeGeorge Mason University
InventorsCaroline Dieckmann Hoemann, Julia Alexa Leonard, Virginia A. Espina, David Kepplinger
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Abstract
An embodiment of the invention provides a method of generating, ex vivo production of soluble Lox-1 (sLox-1), comprising: introducing a sample containing a blood free from an anti-coagulant factor into a device; adding a coagulation enhancing material in the sample; incubating the device; forming a cultured blood clot in the device; and shedding of the sLox-1 outside the cultured blood clot, wherein the method is configured to shed sLox-1 more than an anti-coagulated blood.
Claims25
A method of generating, ex vivo production of soluble Lox-1 (sLox-1), comprising:
- introducing a sample containing blood into a device;
- adding a coagulation enhancing material in the sample;
- incubating a cultured blood clot in the device at a temperature greater than 25° C. and less than 45° C. for at least 2 hours
- and
- shedding of the sLox-1 outside the cultured blood clot, wherein the method is configured to shed sLox-1 more than an anti-coagulated blood.
A device comprising a vacutainer tube and a means of heating to maintain temperature of the device at about 25° C. to 45° C., wherein the device is configured to screen agents that promote or inhibit one or more of the following:
- cell apoptosis, scramblase activity, flippase activity, ADAM17 activity, ADAM10 activity, alpha secretase activity, sLox-1 sheddase activity, tumor necrosis factor activation.
Dependencies are inferred from the claim text.