Wheat stripe resistance genes and method of use
AssigneeThe Sainsbury Laboratory
InventorsMatthew James Moscou, Andrew Marc Dawson
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Abstract
Compositions and methods for enhancing the resistance of wheat and barley plants to wheat stripe rust caused by Puccinia striiformis f. sp. tritici are provided. The compositions comprise nucleic acid molecules encoding resistance (R) gene products and variants thereof and plants, seeds, and plant cells comprising such nucleic acid molecules. The methods for enhancing the resistance of wheat and barley plants to wheat stripe rust comprise introducing a nucleic acid molecule encoding an R gene product into a wheat or barley plant cell. Additionally provided are methods for using the wheat and barley plants in agriculture to limit wheat stripe rust.
Claims19
An expression cassette or vector comprising a nucleic acid molecule and an operably linked heterologous promoter, wherein the nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 2, 5, 7, 9, 11, 15, 34, or 35;
- (b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 3, 6, 8, 10, or 12;
- (c) a nucleotide sequence having at least 90% nucleotide sequence identity to at least one of the full-length nucleotide sequences set forth in SEQ ID NOS:
- 1, 2, 5, 7, 9, and 11, wherein the nucleic acid molecule is capable of conferring resistance to wheat stripe rust to a plant comprising the nucleic acid molecule;
- and
- (d) a nucleotide sequence encoding a polypeptide having at least 90% amino acid sequence identity to at least one of the full-length amino acid sequences set forth in SEQ ID NOS:
- 3, 6, 8, 10, 12, and 14, wherein the nucleic acid molecule is capable of conferring resistance to wheat stripe rust to a plant comprising the nucleic acid molecule.
A method for producing a plant with enhanced resistance to a rust disease caused by a pathogen in the genus Puccinia, the method comprising introducing a heterologous polynucleotide construct into at least one plant cell and regenerating the at least one plant cell into a plant comprising in its genome the heterologous polynucleotide construct, the heterologous polynucleotide construct comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1, 2, 5, 7, 9, or 11;
- (b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 3, 6, 8, 10, or 12;
- (c) a nucleotide sequence having at least 90% nucleotide sequence identity to at least one of the full-length nucleotide sequences set forth in SEQ ID NOS:
- 1, 2, 5, 7, 9, and 11, wherein the nucleic acid molecule is capable of conferring resistance to the plant disease to a plant comprising the nucleic acid molecule;
- and
- (d) a nucleotide sequence encoding a polypeptide having at least 90% amino acid sequence identity to at least one of the full-length amino acid sequences set forth in SEQ ID NOS:
- 3, 6, 8, 10, and 12, wherein the nucleic acid molecule is capable of conferring resistance to the plant disease to a plant comprising the nucleic acid molecule.
A method for producing a barley plant with enhanced resistance to wheat stripe rust, the method comprising modifying in a barley plant or at least one cell thereof, a non-functional allele of the resistance gene Rps6 so as to make a functional allele, whereby the resistance of the barley plant to wheat stripe rust is enhanced, wherein the functional allele comprises a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1;
- and
- (b) a nucleotide sequence having at least 95% nucleotide sequence identity to the full-length nucleotide sequence set forth in SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.