# US 2023/0416773 A1 · Herbicide tolerance genes and methods of use thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2023/0416773 A1 - Document ID: US20230416773A1 - Status: Published application - Kind: A1 - Application number: 18/301,106 - Filed: 2023-04-14 - Published: 2023-12-28 - Earliest claimed: 2014-10-15 - Assignees: Monsanto Technology LLC - Applicants: Monsanto Technology LLC - Inventors: Christine M. Ellis; Artem G. Evdokimov; Paul C.C. Feng; Xiaoran Fu; Clayton T. Larue; Jeffrey R. Nageotte; Andrew C. Read; Lei Shi; Andrew M. Wollacott - Web page: https://patentgrep.com/patent/US20230416773A1 ## CPC - C12N 15/8274: {for herbicide resistance} - A01H 5/10: Seeds - A01N 57/00: Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds - A01N 63/10: Animals; Substances produced thereby or obtained therefrom - C07K 14/195: from bacteria - C12N 9/0071: {acting on paired donors with incorporation of molecular oxygen (1.14)} - C12Y 113/00: Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) - C07K 2319/08: containing a chloroplast localisation signal - C12Y 114/11033: DNA oxidative demethylase (1.14.11.33) - Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - C12Y: ENZYMES - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract Polypeptides and recombinant DNA molecules useful for conferring tolerance to AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides are provided in the present invention, as well as herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same. ## Claims ### Claim at position 1 -20. (canceled) ### Claim 21 A recombinant DNA molecule comprising a nucleic acid sequence encoding a polypeptide having at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52, wherein the polypeptide confers herbicide tolerance to a transgenic plant. ### Claim 22 (depends on 21) The recombinant DNA molecule of claim 21, wherein the nucleic acid sequence is selected from the group consisting of SEQ ID NO:2, 3, 5, 6, 8, 10, 12, 13, 19, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 53-57, and 59. ### Claim 23 (depends on 21) The recombinant DNA molecule of claim 21, wherein the recombinant DNA molecule is operably linked to a heterologous promoter functional in a plant cell. ### Claim 24 (depends on 23) The recombinant DNA molecule of claim 23, wherein the recombinant DNA molecule is further operably linked to a DNA molecule encoding a chloroplast transit peptide. ### Claim 25 (depends on 21) A DNA construct comprising a heterologous promoter functional in a plant cell operably linked to the recombinant DNA molecule of claim 21. ### Claim 26 (depends on 25) The DNA construct of claim 25, further comprising a DNA molecule encoding a chloroplast transit peptide operably linked to the recombinant DNA molecule. ### Claim 27 (depends on 25) The DNA construct of claim 25, wherein the nucleic acid sequence encodes a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52. ### Claim 28 (depends on 25) The DNA construct of claim 25, wherein the DNA construct is present in the genome of a transgenic plant. ### Claim 29 (depends on 21) A plant, seed, plant tissue, plant part, or cell comprising the recombinant DNA molecule of claim 21. ### Claim 30 (depends on 29) The plant, seed, plant tissue, plant part, or cell of claim 29, wherein the plant, seed, plant tissue, plant part, or cell comprises tolerance to at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides. ### Claim 31 (depends on 25) A plant, seed, plant tissue, plant part, or cell comprising the DNA construct of claim 25. ### Claim 32 (depends on 21) A plant, seed, plant tissue, plant part, or cell comprising the polypeptide encoded by the recombinant DNA molecule of claim 21. ### Claim 33 A polypeptide having at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52, wherein the polypeptide confers herbicide tolerance to a transgenic plant. ### Claim 34 (depends on 33) The polypeptide of claim 33, wherein the polypeptide has oxygenase activity against at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides. ### Claim 35 (depends on 33) A method for conferring herbicide tolerance to a plant, seed, cell, or plant part comprising expressing in said plant, seed, cell, or plant part the polypeptide of claim 33. ### Claim 36 (depends on 35) The method of claim 35, wherein said plant, seed, cell, or plant part comprises a DNA construct comprising a heterologous promoter functional in a plant cell operably linked to a recombinant DNA molecule comprising a nucleic acid sequence encoding a polypeptide having at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52. ### Claim 37 (depends on 35) The method of claim 35, wherein the plant, seed, cell, or plant part comprises tolerance to at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides. ### Claim 38 A method for producing an herbicide tolerant transgenic plant comprising transforming a plant cell or tissue with a recombinant DNA molecule comprising a nucleic acid sequence selected from the group consisting of: a) a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52; b) a nucleic acid sequence selected from the group consisting of SEQ ID NO:2, 3, 5, 6, 8, 10, 12, 13, 19, 20, 21, 23, 24, 26, 27, 29, 30, 32, 33, 35, 36, 38, 39, 41, 42, 44, 45, 53-57, and 59; and c) a nucleic acid sequence encoding a polypeptide having at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52, and capable of conferring herbicide tolerance to a transgenic plant; and regenerating an herbicide tolerant transgenic plant from the transformed plant cell or tissue. ### Claim 39 (depends on 38) The method of claim 38, wherein said herbicide tolerant transgenic plant comprises tolerance to at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides. ### Claim 40 A method for controlling weeds in a plant growth area, comprising contacting a plant growth area comprising a plant or seed comprising a recombinant DNA molecule comprising a nucleic acid sequence encoding a protein having at least 90% identity to an amino acid sequence selected from the group consisting of SEQ ID NO:1, 4, 7, 9, 11, 18, 22, 25, 28, 31, 34, 37, 40, 43, 46-50, and 52 with at least one herbicide selected from the group consisting of AOPP herbicides, phenoxy acid herbicides, and pyridinyloxy acid herbicides, wherein the plant or seed is tolerant to the at least one herbicide.